Preface...Table of Contents...Contributing Authors...
1. Measurement of
Staphylococcus aureus Pigmentation by Methanol ExtractionLeia E. Sullivan and Kelly C. Rice
2. Analysis of Murein Hydrolases and Proteases through ZymographyNichole A. Seawell and Jeffrey L. Bose
3. Detection and Quantification of Secreted Nuclease Activity in
Staphylococcus aureus Culture SupernatantsRichard E. Wiemels, Rebecca A. Keogh, and Ronan K. Carroll
4. Quantitative Hemolysis AssaysMiranda J. Ridder, Seth M. Daly, Pamela R. Hall, and Jeffrey L. Bose
5. In Vitro Assay for Quantifying Clumping of
Staphylococcus aureus Heidi A. Crosby, Jakub M. Kwiecinski, and Alexander R. Horswill
6. Measuring Staphylococcal Promoter Activities using a Codon-Optimized β
-galactosidase ReporterChristina N. Krute, Nichole A. Seawell, and Jeffrey L. Bose
7. Evaluation of
Staphylococcus aureus Antibiotic Tolerance using Kill Curve AssaysJessica N. Brandwein and Kelly C. Rice
8. Fluorescence Polarization (FP) Assay for Measuring
Staphylococcus aureus Membrane FluidityKiran B. Tiwari, Suranjana Sen, Craig Gatto, and Brian J. Wilkinson
9. Quantification of
Staphylococcus aureus Biofilm Formation by Crystal Violet and Confocal Microscopy Adam B. Grossman, Dylan J. Burgin, and Kelly C. Rice
10. Growth of
Staphylococcus aureus using a Rotary Cell Culture System Matthew R. Hauserman and Kelly C. Rice
11. Time-Resolved Fluorescence Assay for Measuring Oxygen Consumption Rates in
Staphylococcus aureusLogan L. Bulock, Jennifer L. Endres, and Marat R. Sadykov
12. Quantifying
Staphylococcus aureus Membrane Potential using Flow CytometryNeal D. Hammer
13. Best Practices for Preparation of
Staphylococcus aureus Metabolomics Samples Kimberly L. James and Kelly C. Rice
14. A Rat Model of Orthopedic Implant-Associated Infection for Identification of Staphylococcal Biofilm ProteinsMei G. Lei, Ravi Kr. Gupta, and Chia Y. Lee
15. An Ex Vivo Model for Assessing Growth and Survivability of
Staphylococcus aureus in Whole Human Blood Brittney D. Gimza, Stephanie M. Marroquin, and Lindsey N. Shaw
16. Bone Marrow-Derived Macrophage Infection AssayMiranda J. Ridder, Mary A. Markiewicz, and Jeffrey L. Bose
17. Measurement of Osteoblast Cytotoxicity Induced by S. aureus Secreted ToxinsCaleb Ford and James E. Cassat
18. Human Skin In Vitro Colonization Model for a Skin Wound Infected by
Staphylococcus aureus BiofilmJenelle Chapman and Michael E. Olson
Subject Index List...