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Kniha AMPK Dietbert Neumann

AMPK

Methods and Protocols

Autor: Dietbert Neumann, Benoit Viollet
Jazyk: Angličtina
Vazba: Brožovaná
Vydavatel: Humana Press Inc.
Dostupnost: U nakladatele na objednávku
Odesíláme za 17-27 dnů
3 755 Kč
This volume explores the latest methods used to study AMPK by computational, biochemical, biophysica...

Informace o knize

Autor
Dietbert Neumann, Benoit Viollet
Jazyk
Angličtina
Vazba
Kniha - Brožovaná
Vydáno
2019
Stránek
626
EAN
9781493992492
Enbook ID
21295250
Vydavatel
Humana Press Inc.
Hmotnost
1208
Rozměry
178 x 254 x 254

Kompletní popis

This volume explores the latest methods used to study AMPK by computational, biochemical, biophysical, cellular, and ex vivo and in vivo approaches. The chapters in this book cover specific topics, such as methods to measure change in cellular energy metabolism and analyze metabolic pathways regulated by AMPK; bioinformatics tools to identify AMPK targets; knockdown of AMPK by CRISPR-Cas9; production and crystallization of full-length human AMP-activated protein kinase; cell-free assays to measure the effects of regulatory ligands on AMPK; use of sensors of AMPK activity; AMPK protein interaction by yeast two-hybrid; the role of AMPK in inflammation and autophagy; analyzing the AMPK function in C. elegans and mammals (with special focus on skeletal muscle, blood vessels, kidneys, pancreatic islets and hypothalamus); and human Gamma2 AMPK mutations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, AMPK: Methods and Protocols is a valuable resource for graduate students, postdoctoral researchers, and established investigators who are interested in the richly diverse AMPK field.

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