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Kniha cDNA Library Protocols Ian G. Cowell

cDNA Library Protocols

Autor: Ian G. Cowell, Caroline A. Austin
Jazyk: Angličtina
Vazba: Brožovaná
Vydavatel: Humana Press Inc.
Dostupnost: Skladem u dodavatele
Odesíláme za 5-8 dnů
3 426 Kč
The first libraries of complementary DNA (cDNA) clones were con structed in the mid-to-late 1970s us...

Informace o knize

Autor
Ian G. Cowell, Caroline A. Austin
Jazyk
Angličtina
Vazba
Kniha - Brožovaná
Vydáno
2013
Stránek
321
EAN
9781489940506
ISBN
1489940502
Enbook ID
05182141
Vydavatel
Humana Press Inc.
Hmotnost
486
Rozměry
153 x 230 x 18

Kompletní popis

The first libraries of complementary DNA (cDNA) clones were con structed in the mid-to-late 1970s using RNA-dependent DNA polymerase (reverse transcriptase) to convert poly A mRNA into double-stranded cDNA suitable for insertion into prokaryotic vectors. Since then cDNA technology has become a fundamental tool for the molecular biologist and at the same time some very significant advances have occurred in the methods for con structing and screening cDNA libraries. It is not the aim of cDNA Library Protocols to give a comprehensive review of all cDNA library-based methodologies; instead we present a series of up-to-date protocols that together should give a good grounding of proce dures associated with the construction and use of cDNA libraries. In deciding what to include, we endeavored to combine up-to-date versions of some of the most widely used protocols with some very usefiil newer techniques. cDNA Library Protocols should therefore be especially useful to the investigator who is new to the use of cDNA libraries, but should also be of value to the more experienced worker. Chapters 1 5 concentrate on cDNA library construction and manipula tion, Chapters 6 and 7 describe means of cloning difficult-to-obtain ends of cDNAs, Chapters 8-18 give various approaches to the screening of cDNA libraries, and the remaining chapters present methods of analysis of cDNA clones including details of how to analyze cDNA sequence data and how to make use of the wealth of cDNA data emerging from the human genome project.

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