Kniha Fed-Batch Fermentation Garner G. Moulton

Fed-Batch Fermentation

Jazyk: Angličtina
Vazba: Pevná
Vydavatel: Elsevier Books
Dostupnost: U nakladatele na objednávku
Odesíláme za 28-34 dnů
3 947
Fed-batch fermentation is primarily a practical guide for recombinant protein production in E. coli...

Informace o knize

Jazyk
Angličtina
Vazba
Kniha - Pevná
Vydáno
2013
Stránek
188
EAN
9781907568923
ISBN
1907568921
Enbook ID
05027888
Vydavatel
Hmotnost
442
Rozměry
237 x 164 x 16

Kompletní popis

Fed-batch fermentation is primarily a practical guide for recombinant protein production in E. coli using a fed-batch fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - fed-batch fermentation process can be accomplished within a single day with minimal supervision. This process can also be done on a small (2L) scale that is scalable to 30L or more. All reagents (media, carbon source, plasmid vector and host cell) used are widely available and are relatively inexpensive. This method has been used to produce three different protein products following cGMP guidelines for Phase I clinical studies.

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