New Algorithms for Single Particle Cryo Electron Microscopic Image Processing

Name: New Algorithms for Single Particle Cryo Electron Microscopic Image Processing
Brand: Cuvillier Verlag
SKU: 9783954049073
Price: 728 CZK
Availability: InStock

Autor: Jan-Martin Kirves

Jazyk:

Vazba: Brožovaná

Vydavatel: Cuvillier Verlag

Dostupnost: Skladem u dodavatele

Odesíláme za 8-11 dnů

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In this thesis four approaches to increase the quality and assure the validity of three-dimensional...

Informace o knize

Autor

Jan-Martin Kirves

Jazyk

Angličtina

Vazba

Kniha - Brožovaná

Vydáno

2015

Stránek

174

EAN

9783954049073

ISBN

3954049074

Enbook ID

12828738

Vydavatel

Cuvillier Verlag

Hmotnost

234

Rozměry

148 x 210 x 9

Kompletní popis

In this thesis four approaches to increase the quality and assure the validity of three-dimensional (3D) models generated within the single particle cryo-electron microscopy (CryoEM) workflow are introduced. ProteoPlex is a high-throughput technique and software tool for the evaluation of the most stabilizing biochemical conditions of macromolecular complexes. The validation technique 3D correlation of tilted pairs (3DCTP) can be applied alongside the single particle refinement cycle to detect model bias utilizing tilted pair images. A new analysis method identifies the presence of overfitting in high resolution CryoEM 3D structures. Finally, a set of criteria covering different model quality aspects of the generated 3D structure is introduced.

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New Algorithms for Single Particle Cryo Electron Microscopic Image Processing

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Kompletní popis

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