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Polymerase Chain Reaction

Name: Polymerase Chain Reaction
Brand: LAP LAMBERT Academic Publishing
SKU: 9786200091253
Price: 1200 CZK
Availability: InStock

Autor: Nishant Singh, Kanika Sharma

Jazyk:

Vazba: Brožovaná

Vydavatel: LAP LAMBERT Academic Publishing

Dostupnost: Skladem u dodavatele

Odesíláme za 8-11 dnů

1 200 Kč

Polymerase chain reaction (PCR) is a laboratory technique used to amplify DNA sequences. The method...

Informace o knize

Autor

Nishant Singh, Kanika Sharma

Jazyk

Angličtina

Vazba

Kniha - Brožovaná

Vydáno

2022

Stránek

112

EAN

9786200091253

ISBN

6200091250

Enbook ID

39174847

Vydavatel

LAP LAMBERT Academic Publishing

Hmotnost

185

Rozměry

150 x 220 x 7

Kompletní popis

Polymerase chain reaction (PCR) is a laboratory technique used to amplify DNA sequences. The method involves using short DNA sequences called primers to select the portion of the genome to be amplified. The temperature of the sample is repeatedly raised and lowered to help a DNA replication enzyme copy the target DNA sequence. The technique can produce a billion copies of the target sequence in just a few hours. PCR is a revolutionary method developed by Kary Mullis in the 1980s. PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand. Because DNA polymerase can add a nucleotide only onto a preexisting 3'-OH group, it needs a primer to which it can add the first nucleotide. This requirement makes it possible to delineate a specific region of template sequence that the researcher wants to amplify. At the end of the PCR reaction, the specific sequence will be accumulated in billions of copies (amplicons). PCR is used in many areas of biology and medicine, including molecular biology research, medical diagnostics, and even some branches of ecology.