Kniha Signal Transduction - Single Cell Techniques Bert

Signal Transduction - Single Cell Techniques

Jazyk: Angličtina
Vazba: Brožovaná
Dostupnost: Skladem u dodavatele
Odesíláme za 8-11 dnů
2 234
A variety of powerful techniques for monitoring and analysing events during signal transduction at t...

Informace o knize

Jazyk
Angličtina
Vazba
Kniha - Brožovaná
Vydáno
2012
Stránek
468
EAN
9783642489761
ISBN
3642489761
Enbook ID
02012187
Hmotnost
969
Rozměry
189 x 246 x 27

Kompletní popis

A variety of powerful techniques for monitoring and analysing events during signal transduction at the single cell level are described in this lab manual. An introductionary section on cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. The electrophysiological section illustrates multiple applications of the patch-clamp technique in various cell types from both animals and plants. Emphasis is put on calibration and validation of the different techniques to measure changes of membrane potential, and intracellular ion concentration or pH.

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