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Kniha Two-Hybrid Systems Paul N. MacDonald

Two-Hybrid Systems

Methods and Protocols

Autor: Paul N. MacDonald
Jazyk: Angličtina
Vazba: Brožovaná
Vydavatel: Humana Press Inc.
Dostupnost: Skladem u dodavatele v malém množství
Odesíláme za 11-15 dnů
2 567 Kč
Paul N. MacDonald has assembled a collection of powerful molecular tools for examining and character...

Informace o knize

Autor
Paul N. MacDonald
Jazyk
Angličtina
Vazba
Kniha - Brožovaná
Vydáno
2001
Stránek
336
EAN
9780896038080
ISBN
0896038084
Enbook ID
01400854
Vydavatel
Humana Press Inc.
Hmotnost
535
Rozměry
152 x 229 x 234

Kompletní popis

Paul N. MacDonald has assembled a collection of powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. The techniques range from the most basic (introducing plasmids into yeasts, interaction assays, and recovering the plasmids from yeast), to the most advanced alternative strategies (involving one-hybrid, split two-hybrid, three-hybrid, membrane recruitment systems, and mammalian systems). Methods are also provided for dealing with the well-known problem of artifacts and false positives and for identifying the interacting partners in important biological systems, including the SMAD and nuclear receptor pathways. To ensure ready reproducibility and robust results, each technique is described in step-by-step detail by researchers who employ it regularly.

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