Trichoderma Reesei: Methods and Protocols

1. Ecological Genomics and Evolution of Trichoderma reesei

Komal Chenthamara, Irina S. Druzhinina, Mohammad J. Rahimi, Marica Grujic, and Feng Cai

2. Industrial Relevance of Trichoderma reesei as an Enzyme Producer

Amanda J. Fischer, Suchindra Maiyuran, and Debbie S. Yaver

3. The Potential of Synthetic Biology for Trichoderma reesei

Roland Martzy and Astrid R. Mach-Aigner

4. Resistance Marker- and Gene Gun-Mediated Transformation of Trichoderma reesei

Monika Schmoll and Susanne Zeilinger

5. Use of Auxotrophic Markers for Targeted Gene Insertions in Trichoderma reesei

Irene Tomico-Cuenca and Christian Derntl

6. Open the Pores: Electroporation for the Transformation of Trichoderma reesei

Franziska Wanka

7. Sexual Crossing of Trichoderma reesei

Rita B. Linke

8. CRISPR/Cas9-Mediated Genome Editing of Trichoderma reesei

Gen Zou and Zhihua Zhou

9. The Copper-Controlled RNA Interference System in Trichoderma reesei

Lei Wang, Weixin Zhang, Xiangfeng Meng, and Weifeng Liu

Birgit Jovanovic

11. Image Analysis Method for the Characterization of Trichoderma reesei during Fermentations

Nicolas Hardy, Maxime Moreaud, and Fadhel Ben Chaabane

12. Measurement of Cellulase and Xylanase Activities in Trichoderma reesei

Qing-Shan Meng, Fei Zhang, Chen-Guang Liu, Feng-Wu Bai, and Xin-Qing Zhao

13. Flow Cytometry for Filamentous Fungi

Matthias G. Steiger

14. Molecular Identification of Trichoderma reesei

Mohammad Rahimi, Feng Cai, Marica Grujic, Komal Chenthamara, and Irina S. Druzhinina

15. In Vivo Footprinting Analysis in Trichoderma reesei

Alice Rassinger

16. RNA Characterization in Trichoderma reesei

Petra Till

17. Proteomic Profiling of the Secretome of Trichoderma reesei

SoFong Cam Ngan and Siu Kwan Sze

18. Transcriptomics in Trichoderma reesei

Amanda C.C. Antonieto and Roberto N. Silva

19. The Comprehensive and Reliable Detection of Secondary Metabolites in Trichoderma reesei: A Tool for the Discovery of Novel Substances

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