Bacterial Gene Expression Technologies

Name: Bacterial Gene Expression Technologies
Brand: LAP Lambert Academic Publishing
SKU: 9786202004787
Price: 984 CZK
Availability: InStock

Autor: Mahmoud Zaky, Merna Abou-Selima

Jazyk:

Vazba: Brožovaná

Vydavatel: LAP Lambert Academic Publishing

Dostupnost: Skladem u dodavatele

Odesíláme za 5-8 dnů

984 Kč

Random integration of the IVET vector into the pathogen chromosome is performed by insertion-duplica...

Informace o knize

Autor

Mahmoud Zaky, Merna Abou-Selima

Jazyk

Angličtina

Vazba

Kniha - Brožovaná

Vydáno

2017

Stránek

124

EAN

9786202004787

Enbook ID

18038012

Vydavatel

LAP Lambert Academic Publishing

Hmotnost

203

Rozměry

150 x 220 x 7

Kompletní popis

Random integration of the IVET vector into the pathogen chromosome is performed by insertion-duplication mutagenesis to create a pool of recombinant pathogens (this means that the gene in which the vector has inserted by homologous recombination is not disrupted). Recombinants can be selected using antibiotic resistance, as an additional marker is also on the integrated IVET construct. Pooled clones are then inoculated into the mouse. The two main types of IVET promoter trap strategies are (a) the complementation of auxotrophic mutation and (b) the expression of antibiotic resistance. Only those bacteria that contain the selective marker fused to a gene that is transcriptionally active in the host are able to survive. After a suitable infection period, bacteria that express the marker are isolated from lung, liver, spleen, and mesenteric tissue.

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Bacterial Gene Expression Technologies

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Kompletní popis

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